Evidence for the interaction between SMAD4_HUMAN and SKI_HUMAN



  • 12857746 (Direct interaction of Ski with either Smad3 or Smad4 is necessary and sufficient for Ski-mediated repression of transforming growth factor-beta signaling.; Ueki N, Hayman MJ; 2003)
  • 12419246 (Structural mechanism of Smad4 recognition by the nuclear oncoprotein Ski: insights on Ski-mediated repression of TGF-beta signaling.; Wu JW, Krawitz AR, Chai J, Li W, Zhang F, Luo K, Shi Y; 2002)
  • 12874272 (Requirement of the co-repressor homeodomain-interacting protein kinase 2 for ski-mediated inhibition of bone morphogenetic protein-induced transcriptional activation.; Harada J, Kokura K, Kanei-Ishii C, Nomura T, Khan MM, Kim Y, Ishii S; 2003)
  • 10531062 (Negative feedback regulation of TGF-beta signaling by the SnoN oncoprotein.; Stroschein SL, Wang W, Zhou S, Zhou Q, Luo K; 1999)
  • 14525983 (DACH1 inhibits transforming growth factor-beta signaling through binding Smad4.; Wu K, Yang Y, Wang C, Davoli MA, D'Amico M, Li A, Cveklova K, Kozmik Z, Lisanti MP, Russell RG, Cvekl A, Pestell RG; 2003)
  • 15231748 (Functional proteomics mapping of a human signaling pathway.; Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM; 2004)
  • 12732634 (The oncoprotein Ski acts as an antagonist of transforming growth factor-beta signaling by suppressing Smad2 phosphorylation.; Prunier C, Pessah M, Ferrand N, Seo SR, Howe P, Atfi A; 2003)
  • 19032343 (Ski co-repressor complexes maintain the basal repressed state of the TGF-beta target gene, SMAD7, via HDAC3 and PRMT5.; Tabata T, Kokura K, Ten Dijke P, Ishii S; 2008)
  • 14699069 (Interaction with Smad4 is indispensable for suppression of BMP signaling by c-Ski.; Takeda M, Mizuide M, Oka M, Watabe T, Inoue H, Suzuki H, Fujita T, Imamura T, Miyazono K, Miyazawa K; 2003)
  • 20959473 (The oncoprotein c-ski functions as a direct antagonist of the transforming growth factor-{beta} type I receptor.; Ferrand N, Atfi A, Prunier C; 2010)
  • 15761153 (High-throughput mapping of a dynamic signaling network in mammalian cells.; Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL; 2005)
  • 12764135 (The transforming activity of Ski and SnoN is dependent on their ability to repress the activity of Smad proteins.; He J, Tegen SB, Krawitz AR, Martin GS, Luo K; 2003)
  • 17469184 (Involvement of the constitutive complex formation of c-Ski/SnoN with Smads in the impaired negative feedback regulation of transforming growth factor beta signaling in scleroderma fibroblasts.; Jinnin M, Ihn H, Mimura Y, Asano Y, Tamaki K; 2007)
  • 25609649 (Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.; Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J; 2015)
  • 25670202 (Ski regulates Hippo and TAZ signaling to suppress breast cancer progression.; Rashidian J, Le Scolan E, Ji X, Zhu Q, Mulvihill MM, Nomura D, Luo K; 2015)
  • 28468752 (USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.; Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, van Dinther M, Sixma TK, Huang H, Zhang L, Ten Dijke P; 2017)
  • 26977879 (Inhibiting Drivers of Non-mutational Drug Tolerance Is a Salvage Strategy for Targeted Melanoma Therapy.; Smith MP, Brunton H, Rowling EJ, Ferguson J, Arozarena I, Miskolczi Z, Lee JL, Girotti MR, Marais R, Levesque MP, Dummer R, Frederick DT, Flaherty KT, Cooper ZA, Wargo JA, Wellbrock C; 2016)

Experimental systems

TechniquePSI-MI codeScore
x-ray crystallographyMI:01141
Reconstituted Complex1
Co-crystal Structure1
Affinity Capture-Western0.5
Affinity Capture-MS0.5
tandem affinity purificationMI:06760.5
affinity chromatography technologyMI:00040.5
anti tag coimmunoprecipitationMI:00070.5
anti bait coimmunoprecipitationMI:00060.5
pull downMI:00960.25
cosedimentation through density gradientMI:00290.2
in vivoMI:04930.1
in vitroMI:04920.1

other species

  • None