PSI-MI Method Name Score MI:0369 lex-a dimerization assay 5 MI:0054 fluorescence-activated cell sorting 1 MI:0045 experimental interaction detection 1 MI:0428 imaging technique 1 MI:0091 chromatography technology 1 MI:0051 fluorescence technology 1 MI:0008 array technology 3 MI:0892 solid phase assay 1 MI:0426 light microscopy 1 MI:0090 protein complementation assay 5 MI:0114 x-ray crystallography 10 MI:0065 isothermal titration calorimetry 10 MI:0067 light scattering 10 MI:0077 nuclear magnetic resonance 10 MI:0107 surface plasmon resonance 10 MI:0826 x ray scattering 9 MI:0011 beta lactamase complementation 5 MI:0010 beta galactosidase complementation 5 MI:0440 saturation binding 7.5 MI:0809 bimolecular fluorescence complementation 6 MI:0012 bioluminescence resonance energy transfer 6 MI:0016 circular dichroism 9 MI:0111 dihydrofolate reductase reconstruction 6 MI:0370 tox-r dimerization assay 5 MI:0728 gal4 vp16 complementation 5 MI:0399 two hybrid fragment pooling approach 5 MI:0510 homogeneous time resolved fluorescence 7 MI:0420 kinase homogeneous time resolved fluorescence 7.5 MI:0017 classical fluorescence spectroscopy 7.5 MI:0053 fluorescence polarization spectroscopy 10 MI:0055 fluorescent resonance energy transfer 6 MI:0231 mammalian protein protein interaction trap 6 MI:0099 scintillation proximity assay 7.5 MI:0232 transcriptional complementation assay 5 MI:0112 ubiquitin reconstruction 5 MI:0052 fluorescence correlation spectroscopy 10 MI:0030 cross-linking study 5 MI:0031 protein cross-linking with a bifunctional reagent 5 MI:0084 phage display 6 MI:0048 filamentous phage display 6 MI:0108 t7 phage display 6 MI:0066 lambda phage display 6 MI:0115 yeast display 5 MI:0404 comigration in non denaturing gel electrophoresis 3 MI:0808 comigration in sds page 3 MI:0807 comigration in gel electrophoresis 3 MI:0417 footprinting 3 MI:0089 protein array 5 MI:0424 protein kinase assay 7.5 MI:0434 phosphatase assay 7.5 MI:0415 enzymatic study 1 MI:0411 enzyme linked immunosorbent assay 5 MI:0515 methyltransferase assay 7.5 MI:0516 methyltransferase radiometric assay 7.5 MI:0406 deacetylase assay 7.5 MI:0419 gtpase assay 7.5 MI:0870 demethylase assay 7.5 phosphotransfer assay 7.5 acetylation assay 7.5 MI:0020 transmission electron microscopy 5 MI:0953 polymerization 5 MI:0588 hybrid method 5 MI:0405 competition binding 5 MI:0038 dynamic light scattering 9 MI:0040 electron microscopy 5 MI:0423 in-gel kinase assay 7.5 MI:0726 reverse two hybrid 5 MI:0081 peptide array 5 MI:0006 anti bait coimmunoprecipitation 5 MI:0007 anti tag coimmunoprecipitation 5 MI:0096 pull down 2.5 MI:0676 tandem affinity purification 5 MI:0019 coimmunoprecipitation 5 MI:0059 gst pull down 5 MI:0004 affinity chromatography technology 5 MI:0071 molecular sieving 2 MI:0049 filter binding 5 MI:0029 cosedimentation through density gradient 2 MI:0226 ion exchange chromatography 3 MI:0028 cosedimentation in solution 2 MI:0027 cosedimentation 2 MI:0435 protease assay 7.5 MI:0061 his pull down 5 MI:0025 copurification 2 MI:0276 blue native page 3 MI:0069 mass spectrometry studies of complexes 5 MI:0400 affinity technology 5 MI:0227 reverse phase chromatography 1 MI:0678 antibody array 5 MI:0416 fluorescence microscopy 1 MI:0663 confocal microscopy 1 MI:0022 colocalization by immunostaining 1 MI:0021 colocalization by fluorescent probes cloning 1 MI:0413 electrophoretic mobility shift assay 2 MI:0412 electrophoretic mobility supershift assay 2 MI:0402 chromatin immunoprecipitation assay 2 MI:0254 genetic interference 0 MI:0920 ribonuclease assay 7.5 MI:0047 far western blotting 5 MI:0364 inferred by curator 1 MI:0493 in vivo 1 MI:0492 in vitro 1 MI:0018 two hybrid 5 Affinity Capture-Western 5 Reconstituted Complex 10 FRET 6 Protein-peptide 5 Co-localization 1 Affinity Capture-MS 5 Biochemical Activity 5 Affinity Capture-RNA 2 Co-crystal Structure 10 Co-fractionation 1 Protein-RNA 0 Affinity Capture-Luminescence 5 pull-down/mass spectrometry 5 MI:0825 x-ray fiber diffraction 9 MI:0042 electron paramagnetic resonance 9 MI:0872 atomic force microscopy 9 MI:0023 colocalization/visualisation technologies 1 MI:1313 proximity labelling technology 5 MI:0401 biochemical 5 0