PSI-MI Method Name Score New in Version MI:0013 biophysical 10 True MI:0034 display technology 5 True MI:0073 mrna display 6 True MI:0095 proteinchip(r) on a surface-enhanced laser desorption/ionization 5 True MI:0097 reverse ras recruitment system 5 True MI:0104 static light scattering 9 True MI:0225 chromatin immunoprecipitation array 2 True MI:0256 rna interference 0 True MI:0397 two hybrid array 5 True MI:0398 two hybrid pooling approach 5 True MI:0410 3D electron microscopy 10 True MI:0425 kinase scintillation proximity assay 7.5 True MI:0437 protein three hybrid 5 True MI:0512 zymography 7.5 True MI:0605 enzymatic footprinting 3 True MI:0655 lambda repressor two hybrid 5 True MI:0686 unspecified method 0 True MI:0695 sandwich immunoassay 5 True MI:0727 lexa b52 complementation 5 True MI:0729 luminescence based mammalian interactome mapping 5 True MI:0813 proximity ligation assay 6 True MI:0814 protease accessibility laddering 3 True MI:0841 phosphotransferase assay 7.5 True MI:0858 immunodepleted coimmunoprecipitation 5 True MI:0859 force spectroscopy 9 True MI:0880 atpase assay 7.5 True MI:0888 small angle neutron scattering 9 True MI:0889 acetylase assay 7.5 True MI:0894 electron diffraction 9 True MI:0900 p8 filamentous phage display 6 True MI:0905 amplified luminescent proximity homogeneous assay 7 True MI:0916 lexa vp16 complementation 5 True MI:0928 filter trap assay 3 True MI:0943 detection by mass spectrometry 5 True MI:0944 mass spectrometry study of hydrogen/deuterium exchange 6 True MI:0947 bead aggregation assay 3 True MI:0949 gdp/gtp exchange assay 7.5 True MI:0963 interactome parallel affinity capture 5 True MI:0964 infrared spectroscopy 9 True MI:0966 ultraviolet-visible spectroscopy 9 True MI:0968 biosensor 7 True MI:0969 bio-layer interferometry 10 True MI:0976 total internal reflection fluorescence spectroscopy 9 True MI:0979 oxidoreductase assay 7.5 True MI:0982 electrophoretic mobility-based method 2 True MI:0990 cleavage assay 7.5 True MI:0991 lipoprotein cleavage assay 7.5 True MI:0997 ubiquitinase assay 7.5 True MI:0998 deubiquitinase assay 7.5 True MI:1000 hydroxylase assay 7.5 True MI:1002 myristoylase assay 7.5 True MI:1004 palmitoylase assay 7.5 True MI:1005 adp ribosylase assay 7.5 True MI:1007 glycosylase assay 7.5 True MI:1008 sumoylase assay 7.5 True MI:1010 neddylase assay 7.5 True MI:1016 fluorescence recovery after photobleaching 2 True MI:1017 rna immunoprecipitation 0 True MI:1019 protein phosphatase assay 7.5 True MI:1022 field flow fractionation 2 True MI:1024 scanning electron microscopy 5 True MI:1034 nuclease assay 7.5 True MI:1036 nucleotide exchange assay 7.5 True MI:1037 Split renilla luciferase complementation 6 True MI:1038 silicon nanowire field-effect transistor 9 True MI:1086 equilibrium dialysis 7.5 True MI:1103 solution state nmr 10 True MI:1104 solid state nmr 10 True MI:1113 two hybrid bait and prey pooling approach 5 True MI:1138 decarboxylation assay 7.5 True MI:1147 ampylation assay 7.5 True MI:1203 split luciferase complementation 6 True MI:1204 split firefly luciferase complementation 6 True MI:1232 aggregation assay 3 True MI:1235 thermal shift binding 10 True MI:1246 ion mobility mass spectrometry of complexes 6 True MI:1247 microscale thermophoresis 10 True MI:1309 de-ADP-ribosylation assay 7.5 True MI:1311 differential scanning calorimetry 10 True MI:1314 proximity-dependent biotin identification 3 True MI:1320 membrane yeast two hybrid 5 True MI:1356 validated two hybrid 5 True MI:2169 luminiscence technology 1 True MI:2170 bimolecular luminiscence complementation 6 True MI:2171 complemented donor-acceptor resonance energy transfer 6 True MI:2189 avexis 5 True MI:2215 barcode fusion genetics two hybrid 5 True MI:2289 virotrap 5 True MI:2338 electron tomography 9 True MI:2339 electron microscopy 3D single particle reconstruction 10 True MI:2340 electron microscopy 3D helical reconstruction 10 True MI:2404 oxidase assay 7.5 True MI:2413 mammalian membrane two hybrid 6 True MI:2437 holdup assay 6 True phosphotransfer assay 7.5 False acetylation assay 7.5 False Affinity Capture-Western 5 False Reconstituted Complex 10 False FRET 6 False Protein-peptide 5 False Co-localization 1 False Affinity Capture-MS 5 False Biochemical Activity 5 False Affinity Capture-RNA 2 False Co-crystal Structure 10 False Co-fractionation 1 False Protein-RNA 0 False Affinity Capture-Luminescence 5 False pull-down/mass spectrometry 5 False MI:0004 affinity chromatography technology 5 False MI:0006 anti bait coimmunoprecipitation 5 False MI:0007 anti tag coimmunoprecipitation 5 False MI:0008 array technology 3 False MI:0010 beta galactosidase complementation 5 False MI:0011 beta lactamase complementation 5 False MI:0012 bioluminescence resonance energy transfer 6 False MI:0016 circular dichroism 9 False MI:0017 classical fluorescence spectroscopy 7.5 False MI:0018 two hybrid 5 False MI:0019 coimmunoprecipitation 5 False MI:0020 transmission electron microscopy 5 False MI:0021 colocalization by fluorescent probes cloning 1 False MI:0022 colocalization by immunostaining 1 False MI:0023 colocalization/visualisation technologies 1 False MI:0025 copurification 2 False MI:0027 cosedimentation 2 False MI:0028 cosedimentation in solution 2 False MI:0029 cosedimentation through density gradient 2 False MI:0030 cross-linking study 5 False MI:0031 protein cross-linking with a bifunctional reagent 5 False MI:0038 dynamic light scattering 9 False MI:0040 electron microscopy 5 False MI:0042 electron paramagnetic resonance 9 False MI:0045 experimental interaction detection 1 False MI:0047 far western blotting 5 False MI:0048 filamentous phage display 6 False MI:0049 filter binding 5 False MI:0051 fluorescence technology 1 False MI:0052 fluorescence correlation spectroscopy 10 False MI:0053 fluorescence polarization spectroscopy 10 False MI:0054 fluorescence-activated cell sorting 1 False MI:0055 fluorescent resonance energy transfer 6 False MI:0059 gst pull down 5 False MI:0061 his pull down 5 False MI:0065 isothermal titration calorimetry 10 False MI:0066 lambda phage display 6 False MI:0067 light scattering 10 False MI:0069 mass spectrometry studies of complexes 5 False MI:0071 molecular sieving 2 False MI:0077 nuclear magnetic resonance 10 False MI:0081 peptide array 5 False MI:0084 phage display 6 False MI:0089 protein array 5 False MI:0090 protein complementation assay 5 False MI:0091 chromatography technology 1 False MI:0096 pull down 2.5 False MI:0099 scintillation proximity assay 7.5 False MI:0107 surface plasmon resonance 10 False MI:0108 t7 phage display 6 False MI:0111 dihydrofolate reductase reconstruction 6 False MI:0112 ubiquitin reconstruction 5 False MI:0114 x-ray crystallography 10 False MI:0115 yeast display 5 False MI:0226 ion exchange chromatography 3 False MI:0227 reverse phase chromatography 1 False MI:0231 mammalian protein protein interaction trap 6 False MI:0232 transcriptional complementation assay 5 False MI:0254 genetic interference 0 False MI:0276 blue native page 3 False MI:0364 inferred by curator 1 False MI:0369 lex-a dimerization assay 5 False MI:0370 tox-r dimerization assay 5 False MI:0399 two hybrid fragment pooling approach 5 False MI:0400 affinity technology 5 False MI:0401 biochemical 5 False MI:0402 chromatin immunoprecipitation assay 2 False MI:0404 comigration in non denaturing gel electrophoresis 3 False MI:0405 competition binding 5 False MI:0406 deacetylase assay 7.5 False MI:0411 enzyme linked immunosorbent assay 5 False MI:0412 electrophoretic mobility supershift assay 2 False MI:0413 electrophoretic mobility shift assay 2 False MI:0415 enzymatic study 1 False MI:0416 fluorescence microscopy 1 False MI:0417 footprinting 3 False MI:0419 gtpase assay 7.5 False MI:0420 kinase homogeneous time resolved fluorescence 7.5 False MI:0423 in-gel kinase assay 7.5 False MI:0424 protein kinase assay 7.5 False MI:0426 light microscopy 1 False MI:0428 imaging technique 1 False MI:0434 phosphatase assay 7.5 False MI:0435 protease assay 7.5 False MI:0440 saturation binding 7.5 False MI:0492 in vitro 1 False MI:0493 in vivo 1 False MI:0510 homogeneous time resolved fluorescence 7 False MI:0515 methyltransferase assay 7.5 False MI:0516 methyltransferase radiometric assay 7.5 False MI:0588 hybrid method 5 False MI:0663 confocal microscopy 1 False MI:0676 tandem affinity purification 5 False MI:0678 antibody array 5 False MI:0726 reverse two hybrid 5 False MI:0728 gal4 vp16 complementation 5 False MI:0807 comigration in gel electrophoresis 3 False MI:0808 comigration in sds page 3 False MI:0809 bimolecular fluorescence complementation 6 False MI:0825 x-ray fiber diffraction 9 False MI:0826 x ray scattering 9 False MI:0870 demethylase assay 7.5 False MI:0872 atomic force microscopy 9 False MI:0892 solid phase assay 1 False MI:0920 ribonuclease assay 7.5 False MI:0953 polymerization 5 False MI:1313 proximity labelling technology 5 False