logoCABRA (Cluster & Annotate Blast Results Algorithm)

    Mier, P. & Andrade-Navarro, M. CABRA: Cluster and Annotate Blast Results Algorithm. BMC Res Notes, 9(2016), 253. PMID: 27129717.    



QUERY







PARAMETERS

1) Database

2) BLAST e-value

3) SEG (low complexity filter)

4) Minimum %identity in clusters





How does CABRA work?


Step 1: Get query sequence

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Step 2: BLAST query vs database

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[BLAST 2.2.31 package]
Step 3: Cluster BLAST results

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[FastaHerder2 clustering algorithm]
Step 4: Annotate clusters

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[annotations from UniProt version 2015_10]

Results are ready between seconds to less than 5 minutes, depending on the query length and the chosen database.




CABRA

If you want to perform the BLAST search in your own database, and then cluster the results, we provide you with the standalone version of CABRA. Use it wisely: CABRA standalone. Be aware that the functionalities regarding the annotations of the clusters are not present in the standalone version, as we wouldn't have the annotations for the protein sequences from your databases.