dAtabase of Polyx Evolution

Input & output overview

Query: Download

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Clustered results: Download

Ortholog's multifasta file: Download

Ortholog's alignment (fasta format): Download

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Cutoff22: -

In this example, we used as query a mixed set of SMN, SPF30 and RSMN proteins. By default, the multiple sequence alignment of all of them is displayed below (tag "Raw results"), with their homorepeats showcased in different colors. Clicking in the tag "Clustered results", one can see the clustered results, and the sequences correctly distributed in three clusters. The distribution of the homorepeats in the three clusters is indeed very different, and homogeneus within them. To cluster the sequences, we used the standalone version of FastaHerder2 (PMID: 26828375), using a threshold of a minimum of 25% identity in the clusters.

• Raw results
• Clustered results

Ortholog's alignment simplification, showing the position of the polyX regions

polyA --- polyC --- polyD --- polyE --- polyF --- polyG --- polyH --- polyI --- polyK --- polyL --- polyM --- polyN --- polyP --- polyQ --- polyR --- polyS --- polyT --- polyV --- polyW --- polyY ---

	Q16637 P97801 O18870 P63162 O75940 P63164 P63163 Q17QN3 Q5R591 Q7ZV80 Q6DEY1	1 1 1 1 1 1 1 1 1 1 1	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	294 288 287 240 238 240 240 240 238 237 238
			*To visualize the actual sequence of a polyX, please go to the individual table of each amino acid (below). **Alignment represented in scale 1:2.

Ortholog's alignment simplification, showing the position of the polyX regions

polyA --- polyC --- polyD --- polyE --- polyF --- polyG --- polyH --- polyI --- polyK --- polyL --- polyM --- polyN --- polyP --- polyQ --- polyR --- polyS --- polyT --- polyV --- polyW --- polyY ---

	Cluster 1 Q16637 P97801 O18870 Cluster 2 P63164 P63162 Q17QN3 P63163 Cluster 3 Q5R591 Q7ZV80 Q6DEY1 O75940	1 1 1 1 1 1 1 1 1 1 1	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	294 288 287 240 240 240 240 238 237 238 238
			*To visualize the actual sequence of a polyX, please go to the individual table of each amino acid (below). **Alignment represented in scale 1:2.

	Input sequences were clustered with FastaHerder2 (PMID: 26828375), using a threshold of a minimum of 25% identity in the clusters:
	>1-(1:)sp|Q16637|SMN_HUMAN-(1:)sp|O18870|SMN_BOVIN;(1:)sp|P97801|SMN_MOUSE;   >2-(1:)sp|Q17QN3|RSMN_BOVIN-(1:)sp|P63163|RSMN_MOUSE;(1:)sp|P63164|RSMN_RAT;(1:)sp|P63162|RSMN_HUMAN;   >3-(1:)sp|Q7ZV80|SPF30_DANRE-(2:)sp|O75940|SPF30_HUMAN;(2:)sp|Q5R591|SPF30_PONAB;(2:)sp|Q6DEY1|SPF30_XENTR;

click on any graph to show the results per amino acid

polyA

*Numbers on the right refer to the maximum number of A found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 P63162 149-160 119-190 PIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMA 4 5 6 7 8 9 9 9 9 9 P63164 149-160 119-190 PIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMA 4 5 6 7 8 9 9 9 9 9 P63163 149-160 119-190 PIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMA 4 5 6 7 8 9 9 9 9 9 Q17QN3 149-160 119-190 PIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTAPTPVGRATPPPGIMA 4 5 6 7 8 9 9 9 9 9

polyD

*Numbers on the right refer to the maximum number of D found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q6DEY1 59-66 29-96 NEDLLKLKKDLQEVIELTKDLLSSQPSETADDACDDMSASGSQSWKVGEKCMAVWSDDGQWYEAEIEE 2 4 4 4 4 4 4 5 5 5

polyE

*Numbers on the right refer to the maximum number of E found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 O75940 91-102 61-132 SDSFASTQPTHSWKVGDKCMAVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAK 3 4 5 6 6 6 7 7 7 7 Q5R591 91-102 61-132 SDSFASTQPTHSWKVGDKCMAIWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAK 3 4 5 6 6 6 7 7 7 7 Q7ZV80 91-98 61-128 TKSSETVAPSHSWRVGDHCMATWSQDGQVYEAEIEEIDNENGTAAITFAGYGNAEVMPLHMLKKVEEG 3 4 4 5 5 5 5 5 5 5 Q6DEY1 91-102 61-132 ACDDMSASGSQSWKVGEKCMAVWSDDGQWYEAEIEEIDEENGTAAITFAGYGNAEVTSLLNLRPVEEGRKAK 3 4 5 6 6 6 6 6 6 6

polyG

*Numbers on the right refer to the maximum number of G found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q16637 5-13 1-43 MAMSSGGSGGGVPEQEDSVLFRRGTGQSDDSDIWDDTALIKAY 3 5 5 5 5 5 5 5 6 7 P97801 4-10 1-40 MAMGSGGAGSEQEDTVLFRRGTGQSDDSDIWDDTALIKAY 3 4 4 4 4 4 4 5 6 6 O18870 1-9 1-39 MGGGGGGFPEPEDSVLFRRGTGESDDSDVWDDTALIKAY 4 6 6 6 6 6 6 6 7 8 P63162 102-108 72-138 LRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPS 3 4 4 5 6 6 7 7 8 8 P63164 102-108 72-138 LRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPS 3 4 4 5 6 6 7 7 8 8 P63163 102-108 72-138 LRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPS 3 4 4 5 6 6 7 7 8 8 Q17QN3 102-108 72-138 LRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPS 3 4 4 5 6 6 7 7 8 8

polyK

*Numbers on the right refer to the maximum number of K found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 O75940 151-162 121-192 NLKPVEEGRKAKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKG 4 4 6 6 6 7 7 7 8 8 Q5R591 151-162 121-192 NLKPVEEGRKAKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKG 4 4 6 6 6 7 7 7 8 8 Q7ZV80 137-144 107-174 TFAGYGNAEVMPLHMLKKVEEGRIRDEIDGKPKSKKELQAEQREYKKKKAQKKVQRMKELEQEREDQK 4 4 6 6 6 7 7 7 8 9 Q7ZV80 150-161 120-191 HMLKKVEEGRIRDEIDGKPKSKKELQAEQREYKKKKAQKKVQRMKELEQEREDQKSKWQQFNNKAYSKNKKG 4 4 6 6 6 7 7 7 8 9 Q6DEY1 151-162 121-192 NLRPVEEGRKAKEDSGNLPMSKKEMIAAQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNKAYSKNKKG 4 4 6 6 6 7 7 7 8 8

polyL

*Numbers on the right refer to the maximum number of L found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 P63162 67-74 37-104 HMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGP 3 4 4 4 5 5 5 5 5 5 P63164 67-74 37-104 HMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGP 3 4 4 4 5 5 5 5 5 5 P63163 67-74 37-104 HMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGP 3 4 4 4 5 5 5 5 5 5 Q17QN3 67-74 37-104 HMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGP 3 4 4 4 5 5 5 5 5 5

polyP

*Numbers on the right refer to the maximum number of P found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q16637 193-204 163-234 SQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLP 4 6 8 10 10 10 10 11 12 12 Q16637 215-228 185-258 PKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDAD 4 6 8 10 10 10 10 11 12 13 Q16637 240-251 210-281 PGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMGFR 4 6 8 10 10 10 10 11 12 13 P97801 188-197 158-227 SQVSTDDSEHSSRSLRSKAHSKSKAAPWTSFLPPPPPMPGSGLGPGKPGLKFNGPPPPPPLPPPPFLPCW 4 6 7 9 10 11 11 11 11 12 P97801 210-225 180-255 SKAAPWTSFLPPPPPMPGSGLGPGKPGLKFNGPPPPPPLPPPPFLPCWMPPFPSGPPIIPPPPPISPDCLDDTDAL 4 6 7 9 10 11 11 12 13 14 P97801 235-246 205-276 PGLKFNGPPPPPPLPPPPFLPCWMPPFPSGPPIIPPPPPISPDCLDDTDALGSMLISWYMSGYHTGYYMGFR 4 6 7 9 10 11 11 12 13 14 O18870 188-196 158-226 QISTDESENSSRSPLNKPNNIRSRAAPWNSFLPPPPHMPRSGLGPGKSGLNFSGPPPPPPPPPHFLSRW 4 6 8 9 9 9 9 9 10 10 O18870 210-222 180-252 SRAAPWNSFLPPPPHMPRSGLGPGKSGLNFSGPPPPPPPPPHFLSRWLPPFPAGPPMIPPPPPICPDSLDDAD 4 6 8 9 9 9 10 11 12 12 O18870 234-245 204-275 KSGLNFSGPPPPPPPPPHFLSRWLPPFPAGPPMIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMGFK 4 6 8 9 9 9 10 11 12 12 P63162 173-180 143-210 TPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARG 4 4 5 6 7 7 8 9 10 10 P63162 197-204 167-234 QYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPG 4 4 5 7 8 8 9 11 11 12 P63162 220-227 190-240 APPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 P63162 229-235 199-240 MGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 P63164 173-180 143-210 TPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARG 4 4 5 6 7 7 8 9 10 10 P63164 197-204 167-234 QYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPG 4 4 5 7 8 8 9 11 11 12 P63164 220-227 190-240 APPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 P63164 229-235 199-240 MGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 P63163 173-180 143-210 TPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARG 4 4 5 6 7 7 8 9 10 10 P63163 197-204 167-234 QYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPG 4 4 5 7 8 8 9 11 11 12 P63163 220-227 190-240 APPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 P63163 229-235 199-240 MGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 Q17QN3 197-204 167-234 QYPPGRGTAPTPVGRATPPPGIMAPPPGMRPPMGPPIGLPPTRGTPIGMPPPGMRPPPPGIRGPPPPG 4 4 5 7 8 8 9 11 11 12 Q17QN3 220-227 190-240 APPPGMRPPMGPPIGLPPTRGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12 Q17QN3 229-235 199-240 MGPPIGLPPTRGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP 4 4 5 7 8 8 9 11 11 12