dAtabase of Polyx Evolution

Input & output overview

Query: Download

Time elapsed: 1 seconds

Clustered results: Download

Ortholog's multifasta file: Download

Ortholog's alignment (fasta format): Download

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Cutoff22: -

In this example, we used as query a set of Mef2A proteins. By default, the multiple sequence alignment of all of them is displayed below (tag "Raw results"), with their homorepeats showcased in different colors. Clicking in the tag "Clustered results", one can see the clustered results, and the sequences correctly clustered all together (all of them are Mef2A proteins). To cluster the sequences, we used the standalone version of FastaHerder2 (PMID: 26828375), using a threshold of a minimum of 25% identity in the clusters. The polyQ (in blue) and the polyP (in red) regions have varied in length during their evolution, while the polyS regions (in pink, C-terminal) have maintained the same sequence in all of these orthologs ("SLSSSSSS"; it can be seen in the table below with the information of polyS).

• Raw results
• Clustered results

Ortholog's alignment simplification, showing the position of the polyX regions

polyA --- polyC --- polyD --- polyE --- polyF --- polyG --- polyH --- polyI --- polyK --- polyL --- polyM --- polyN --- polyP --- polyQ --- polyR --- polyS --- polyT --- polyV --- polyW --- polyY ---

	Q02078 Q60929 Q2MJT0 Q03414 A2VDZ3 A2ICN5 Q5REW7 Q9W6U8	1 1 1 1 1 1 1 1	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	507 498 495 516 492 507 494 499
			*To visualize the actual sequence of a polyX, please go to the individual table of each amino acid (below). **Alignment represented in scale 1:3.

Ortholog's alignment simplification, showing the position of the polyX regions

polyA --- polyC --- polyD --- polyE --- polyF --- polyG --- polyH --- polyI --- polyK --- polyL --- polyM --- polyN --- polyP --- polyQ --- polyR --- polyS --- polyT --- polyV --- polyW --- polyY ---

	Cluster 1 Q60929 A2ICN5 A2VDZ3 Q2MJT0 Q02078 Q03414 Q9W6U8 Q5REW7	1 1 1 1 1 1 1 1	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	498 507 492 495 507 516 499 494
			*To visualize the actual sequence of a polyX, please go to the individual table of each amino acid (below). **Alignment represented in scale 1:3.

	Input sequences were clustered with FastaHerder2 (PMID: 26828375), using a threshold of a minimum of 25% identity in the clusters:
	>1-(1:)sp|Q03414|MEF2A_XENLA-(1:)sp|Q02078|MEF2A_HUMAN;(1:)sp|A2VDZ3|MEF2A_BOVIN;(1:)sp|Q2MJT0|MEF2A_RAT;(1:)sp|A2ICN5|MEF2A_PIG;(1:)sp|Q5REW7|MEF2A_PONAB;(1:)sp|Q60929|MEF2A_MOUSE;(1:)sp|Q9W6U8|MEF2A_CHICK;

click on any graph to show the results per amino acid

polyE

*Numbers on the right refer to the maximum number of E found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q02078 288-296 258-326 PPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELELNTQRISSSQATQPLATPVVSVTTPSLPPQG 4 5 5 5 5 5 5 5 5 5 Q60929 286-294 256-324 PPGGGSLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELELNAQRISSSQATQPLATPVVSVTTPSLPPQG 4 5 5 5 5 5 5 5 5 5 A2ICN5 288-296 258-326 PPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELELNTQRISSSQAPQPLATPVVSVTTPSLPQQG 4 5 5 5 5 5 5 5 5 5 Q9W6U8 289-295 259-325 GGGGLGMNNRKPDLRVVIPPSSKGMMPPLTEEDELELNTQRISSSQSTQPLATPVVSVTTPSFPPAG 3 4 4 4 4 4 4 4 4 4

polyG

*Numbers on the right refer to the maximum number of G found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q02078 260-265 230-295 VNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELE 3 4 4 4 4 4 4 5 5 5 Q60929 258-263 228-293 VNSRASPNLIGNTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELE 3 4 4 4 4 4 4 5 5 5 Q2MJT0 260-265 230-295 VDSRASPNLIGNTGANSVGKVMPTKSPPPPGGGSVGMNSRKPDLRVVIPPSSKGMMPPLNAQRISS 3 4 4 4 4 4 4 5 5 5 A2VDZ3 260-265 230-295 VNSRASPNLIGTTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPSSKGMMPPLNTQRISS 3 4 4 4 4 4 4 5 5 5 A2ICN5 260-265 230-295 VNSRASPNLVGTTGANSLGKVMPTESPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELE 3 4 4 4 4 4 4 5 5 5 Q5REW7 258-263 228-293 VNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLNTQRISS 3 4 4 4 4 4 4 5 5 5 Q9W6U8 238-248 208-278 GTTDLTVPNGAGTSPVGNGVWNSRASPSLLGTAGGGNGLGKVMPTKSPPPPGGGGLGMNNRKPDLRVVIPP 4 5 5 6 6 6 6 6 7 9 Q9W6U8 257-265 227-295 VWNSRASPSLLGTAGGGNGLGKVMPTKSPPPPGGGGLGMNNRKPDLRVVIPPSSKGMMPPLTEEDELEL 4 5 5 6 6 6 6 6 7 9

polyH

*Numbers on the right refer to the maximum number of H found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q03414 453-459 423-489 LSINTNQNINIKSEPISPPRDRITPSGFQSHQHHQHQPRPEMDSLSSSSSSYDGSDREDVRNDFHSP 3 4 4 4 4 4 4 4 4 4

polyP

*Numbers on the right refer to the maximum number of P found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q02078 254-261 224-291 PVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEE 4 4 5 5 5 5 5 5 6 6 Q02078 429-447 399-477 NISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDF 4 5 6 7 9 10 11 11 11 11 Q60929 130-137 100-167 DADDYFEHSPLSEDRFSKLNEDSDFIFKRGPPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLA 2 4 4 4 4 4 5 5 6 6 Q60929 252-259 222-289 PVGNGFVNSRASPNLIGNTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPSSKGMMPPLSEE 4 4 5 5 5 5 5 5 6 6 Q60929 427-438 397-468 NINIKSEPISPPRDRMTPSGFQQQQQQPQQQPPPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDF 3 5 6 7 7 8 8 8 8 9 Q2MJT0 254-261 224-291 PVGNGFVDSRASPNLIGNTGANSVGKVMPTKSPPPPGGGSVGMNSRKPDLRVVIPPSSKGMMPPLNAQ 4 4 5 5 5 5 5 5 6 6 Q2MJT0 422-435 392-465 INIKTEPISPPRDRMTPSGFQQQQQQQPQQQPPPQPPQPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDF 3 5 6 7 8 8 9 9 9 9 Q03414 294-301 264-331 PVGNGFVNPRASPSHLGPTGGNVLGKVMPTKSPPPPGGNLVMNSRKPDLRVVIPPSSKGMMPPLNTQR 4 4 5 5 5 5 5 5 6 6 A2VDZ3 254-261 224-291 PVGNGFVNSRASPNLIGTTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPSSKGMMPPLNTQ 4 4 5 5 5 5 5 5 6 6 A2VDZ3 416-427 386-457 INTNQNINIKSEPISPPRDRMTPSGFQQQQQPQPPPPPPQAPQPQPRQEVGRSPVDSLSSSSSSYDGSDRED 4 6 7 7 8 9 10 10 10 10 A2ICN5 254-261 224-291 PVGNGFVNSRASPNLVGTTGANSLGKVMPTESPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEE 4 4 5 5 5 5 5 5 6 6 A2ICN5 422-429 392-459 LSINTNQNINIKSEPISPPRDRMTPSGFQQQQPPPPSQAPQPQPPQPQPQPQPQARQEMGRSPVDSLS 4 4 5 6 7 8 9 10 11 12 A2ICN5 431-445 401-475 NIKSEPISPPRDRMTPSGFQQQQPPPPSQAPQPQPPQPQPQPQPQARQEMGRSPVDSLSSSSSSYDGSDREDPRG 4 4 5 6 7 8 9 10 11 12 Q5REW7 130-137 100-167 DADDYFEHSPLSEDRFSKLNEDSDFIFKRGPPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLA 2 4 4 4 4 4 5 5 6 6 Q5REW7 252-259 222-289 PVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLNTQ 4 4 5 5 5 5 5 5 6 6 Q5REW7 417-434 387-464 NQNINIKSEPISPPRDRMTPSGFQQQQQQQQQPPPPPPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDF 4 6 7 8 9 10 11 11 11 11 Q9W6U8 253-260 223-290 VGNGVWNSRASPSLLGTAGGGNGLGKVMPTKSPPPPGGGGLGMNNRKPDLRVVIPPSSKGMMPPLTEE 4 4 5 5 5 5 5 5 6 6 Q9W6U8 427-439 397-469 QNINIKSEPISPPRDRVNSSGFPQQQPPQQPQPPQPPQQPPQRQEMGRSPVDSLSSSSSSYDGSDREDPRSDF 3 4 5 6 7 8 9 9 10 10

polyQ

*Numbers on the right refer to the maximum number of Q found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q02078 418-432 388-462 QGSNLSINTNQNISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSS 4 6 8 10 11 11 11 12 13 14 Q60929 416-429 386-459 QGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQQPQQQPPPQPPQPQPRQEMGRSPVDSLSSSSSSYDGS 4 6 7 9 9 10 10 11 12 13 Q2MJT0 410-424 380-454 QGSNLSINTNQNINIKTEPISPPRDRMTPSGFQQQQQQQPQQQPPPQPPQPQPQPRQEMGRSPVDSLSSSSSSYD 4 6 7 9 10 10 11 12 13 14 A2VDZ3 410-419 380-449 QGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQPQPPPPPPQAPQPQPRQEVGRSPVDSLSSSSSS 4 5 6 6 6 7 7 8 9 10 A2ICN5 418-425 388-455 QGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQPPPPSQAPQPQPPQPQPQPQPQARQEMGRSPV 4 4 4 5 6 7 7 8 9 10 Q5REW7 408-420 378-450 QGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQQQQQPPPPPPQPQPPQPQPRQEMGRSPVDSLSSSSS 4 6 8 9 9 9 10 11 11 12 Q9W6U8 420-429 390-459 NLSINTNQNINIKSEPISPPRDRVNSSGFPQQQPPQQPQPPQPPQQPPQRQEMGRSPVDSLSSSSSSYDG 3 4 5 6 7 7 9 9 10 11

polyS

*Numbers on the right refer to the maximum number of S found per window length in the regions neighboring the homorepeat (± 30 amino acids). Optional values for the different cutoffs apply, and only the homorepeats fulfilling those cutoffs will appear here. ID PolyX position Region position Region with polyX 4 6 8 10 12 14 16 18 20 22 Q02078 456-466 426-496 QQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPS 4 6 7 7 8 8 9 9 9 9 Q60929 447-457 417-487 FQQQQQQPQQQPPPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPS 4 6 7 7 8 8 9 9 9 9 Q2MJT0 444-454 414-484 QQQQQPQQQPPPQPPQPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNAEDRESPS 4 6 7 7 8 8 9 9 9 9 Q03414 465-475 435-505 SEPISPPRDRITPSGFQSHQHHQHQPRPEMDSLSSSSSSYDGSDREDVRNDFHSPIGLGRPANNEDRDSPS 4 6 7 7 8 8 8 8 8 8 A2VDZ3 441-451 411-481 FQQQQQPQPPPPPPQAPQPQPRQEVGRSPVDSLSSSSSSYDGSDREDPRGDFHSPVVLGRPPNSEDRESPS 4 6 7 7 8 8 9 9 9 9 A2ICN5 456-466 426-496 PPSQAPQPQPPQPQPQPQPQARQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPVVLGRPPNTEDRESPS 4 6 7 7 8 8 9 9 9 9 Q5REW7 443-453 413-483 QQQQQQPPPPPPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPS 4 6 7 7 8 8 9 9 9 9 Q9W6U8 169-174 139-204 SMSVTVPVSNPNTLTYSNPGSSLVSPSLAASSSLTSTTMLSPPQTTLHRNVSPGAPQRPPSTGNAG 3 4 4 5 6 7 8 8 9 9 Q9W6U8 300-306 270-336 PDLRVVIPPSSKGMMPPLTEEDELELNTQRISSSQSTQPLATPVVSVTTPSFPPAGLVYSAMPTAYN 3 4 4 4 4 4 5 5 6 6 Q9W6U8 448-458 418-488 FPQQQPPQQPQPPQPPQQPPQRQEMGRSPVDSLSSSSSSYDGSDREDPRSDFHSPVVLGRPPNSEDRESPS 4 6 7 7 8 8 9 9 9 9